Title: Formation of (C center dot G)*G triplets in a B-DNA duplex with overhanging bases
Authors: Vlieghe, D ×
Van Meervelt, Luc
Dautant, A
Gallois, B
Precigoux, G
Kennard, O #
Issue Date: Jan-1996
Publisher: Munksgaard int publ ltd
Series Title: Acta crystallographica section d-biological crystallography vol:52 pages:766-775
Abstract: Crystallization of a DNA double helix with overhanging bases at the 5'-ends of both strands, results in the formation of two crystallographically independent (C . G)*G triplets. In a previous report [Van Meervelt, Vlieghe, Dautant, Gallois, Precigoux & Kennard (1995). Nature (London), 374, 742-744] the unique molecular packing of the duplex and the Hoogsteen hydrogen-bond pattern and parallel backbone orientation of the guanine-containing strands in the triplets was described. The fine structural details and hydration of the d(GCGAATTCG) crystal structure refined to 2.05 Angstrom (R = 0.168, 86 water molecules, two Mg2+ cations) are now presented. Helical parameters, stacking effects, the geometry at the duplex-tripler junction, and the hydration of the minor groove are discussed and compared with related theoretical and crystal structures.
ISSN: 1399-0047
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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