Title: Sudan-beta-D-glucuronides and their use for the histochemical localization of beta-glucuronidase activity in transgenic plants
Authors: Van der Eycken, Erik ×
Terryn, N
Goeman, JL
Carlens, G
Nerinckx, W
Claeyssens, M
Van der Eycken, J
Van Montagu, M
Brito-Arias, M
Engler, G #
Issue Date: Jan-2000
Publisher: Springer-verlag
Series Title: Plant cell reports vol:19 issue:10 pages:966-970
Abstract: Synthesis of five different Sudan-beta -D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroadoyl-alpha -D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used for histochemical localization of beta -glucuronidase (GUS) activity in transgenic plants (Petunia hybrida, Arabidopsis thaliana, and Nicotiana tabacum) that contained the GUS reporter system. Because the cleavage of the beta -glucuronide results in the liberation of an insoluble Sudan dye, Sudan substrates gave no diffusion artifacts as described for the commonly used 5-bromo-4-chloro-3-indolyl-beta -D-glucuronide (X-gluc). A comparison of assays with different Sudan glucuronides and X-gluc demonstrated that the SudanIV variant is a valuable glucuronide substrate for the precise histochemical localization of GUS activity in transgenic plants.
ISSN: 0721-7714
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Design and Synthesis
× corresponding author
# (joint) last author

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