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Title: Probing Live Samples in Second-Harmonic Generation Microscopy Using Specific Markers and Fluorescent Proteins
Authors: De Meulenaere, Evelien
Paesen, Rik
Psilodimitrakopoulos, Sotiris
Ameloot, Marcel
Loza-Alvarez, Pablo
Vanderleyden, Jos
Clays, Koen #
Issue Date: 2012
Publisher: SPIE-INT SOC OPTICAL ENGINEERING
Series Title: Probing Live Samples in Second-Harmonic Generation Microscopy Using Specific Markers and Fluorescent Proteins vol:8226 pages:0 -0
Host Document: Proceedings of SPIE
Conference: SPIE Photonics West, MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XII edition:2012 location:Moscone Center, San Fransisco date:22-24 Jan 2012
Article number: 82263C
Abstract: In an effort to complement cellular two-photon excited fluorescence (TPEF) microscopy with structural information from second-harmonic generation (SHG) imaging, we investigated the applicability of fluorescent proteins for SHG imaging. In the first stage, the first hyperpolarizability beta, a measure for the second-order nonlinear optical properties of a molecule, was determined for several fluorescent proteins. In a second stage, an established HeLa cell line expressing a membrane protein labeled with a fluorescent protein, was adapted and imaged using simultaneous TPEF and SHG microscopy. The contour of stretched cells observed in these experiments was proven to be originating in microtubules instead of the fluorescent proteins.
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Molecular Imaging and Photonics
Centre of Microbial and Plant Genetics
# (joint) last author

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