Title: Pressure- and temperature-induced unfolding and aggregation of recombinant human interferon-gamma: a Fourier transform infrared spectroscopy study
Authors: Goossens, K ×
Haelewyn, J
Meersman, Filip
De Ley, Marc
Heremans, Karel #
Issue Date: Jan-2003
Publisher: Portland press
Series Title: Biochemical journal vol:370 pages:529-535
Abstract: The effect of hydrostatic pressure on the secondary structure of recombinant human interferon-gamma (rhIFN-7) and its biologically inactive truncated form rhIFN-DeltaC15 has been studied using Fourier-transform IR (FTIR) spectroscopy. In situ observation of the pressure-induced changes using the diamond anvil cell shows that the alpha-helical structure is mainly transformed into disordered structure at high pressure. Increasing pressure also induces the formation of a gel. Addition of 0.5 M MgCl2 significantly reduces the pressure stability. Releasing the pressure below 300 MPa results in the formation of intermolecular antiparallel beta-sheets, which is seldom observed. This suggests that the intermolecular beta-sheet of rhIFN-gamma is stabilized by electrostatic interactions that are disrupted at high pressure. For comparison we also studied the effect of temperature. Temperature-induced changes reflect extensive transformation of alpha-helical structure into intermolecular antiparallel beta-sheet, as is usually observed for most proteins.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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