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Title: Automated protein-DNA interaction screening of Drosophila regulatory elements
Authors: Hens, Korneel ×
Feuz, Jean-Daniel
Isakova, Alina
Iagovitina, Antonina
Massouras, Andreas
Bryois, Julien
Callaerts, Patrick
Celniker, Susan E
Deplanckeadenine, Bart #
Issue Date: Dec-2011
Publisher: Nature Pub. Group
Series Title: Nature Methods vol:8 issue:12 pages:1065-+
Abstract: Drosophila melanogaster has one of the best characterized metazoan genomes in terms of functionally annotated regulatory elements. To explore how these elements contribute to gene regulation, we need convenient tools to identify the proteins that bind to them. Here we describe the development and validation of a high-throughput yeast one-hybrid platform, which enables screening of DNA elements versus an array of full-length, sequence-verified clones containing over 85% of predicted Drosophila transcription factors. Using six well-characterized regulatory elements, we identified 33 transcription factor-DNA interactions of which 27 were previously unidentified. To simultaneously validate these interactions and locate the binding sites of involved transcription factors, we implemented a powerful microfluidics-based approach that enabled us to retrieve DNA-occupancy data for each transcription factor throughout the respective target DNA elements. Finally, we biologically validated several interactions and identified two new regulators of sine oculis gene expression and hence eye development.
ISSN: 1548-7091
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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