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Title: Identification of local carboxy-terminal hydrophobic interactions essential for folding or stability of chloramphenicol acetyltransferase
Authors: VanderSchueren, J ×
Robben, Johan
Goossens, K
Heremans, Karel
Volckaert, Guido #
Issue Date: Jan-1996
Publisher: Academic press ltd
Series Title: Journal of Molecular Biology vol:256 issue:5 pages:878-888
Abstract: The role of the carboxy terminal in folding and stabilization of type I chloramphenicol acetyltransferase (CAT(I)) has been studied by mutagenesis and Fourier transform infrared analysis. We have shown that a CAT mutant truncated by seven amino acid residues folds into active protein. In this study, the last three residues of this truncated CAT mutant were randomized to detect structural information required for achieving a native enzyme conformation. Statistical analysis of sequencing data from randomly chosen mutants revealed that the amino-terminal CAT fragment of 212 amino acid residues is the shortest deletion mutant able to adopt a soluble, enzymatically active structure. This minimal length corresponds to a protein with full-length alpha(5)-helix in the three-dimensional crystal structure of CAT type III. The amino acid preferences at the carboxy terminal in the randomization experiments suggest that this helix also forms completely in the shortened CAT mutants. In addition correct folding and/or stabilization requires the formation of a hydrophobic microdomain at the end of the alpha(5)-helix. The role of this hydrophobic interaction in CAT folding and structure stabilization is discussed. (C) 1996 Academic Press Limited
URI: 
ISSN: 0022-2836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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