Title: Dynamics of the linuron hydrolase libA gene pool size in response to linuron application and environmental perturbations in agricultural soil and on-farm biopurification systems
Authors: Bers, Karolien ×
Sniegowski, Kristel
De Mot, René
Springael, Dirk #
Issue Date: 2012
Publisher: American Society for Microbiology (ASM)
Series Title: Applied and Environmental Microbiology vol:78 issue:8 pages:2783-2789
Abstract: libA, a gene encoding a novel type of linuron hydrolase, was recently identified in the linuron-mineralizing Variovorax sp. strain SRS16. In order to assess the contribution of libA to linuron degradation in environmental settings, libA abundance was monitored in response to the application of linuron and to environmental perturbations in agricultural soil microcosms and microcosms simulating the matrix of on-farm biopurification systems. libA numbers were measured by real-time PCR and linked to reported data of Variovorax community composition and linuron mineralization capacity. In the soil microcosms and one biopurification system setup, libA numbers responded to the application of linuron and environmental changes in congruency with the modulation of linuron mineralization capacity and the occurrence of a particular Variovorax phylotype (phylotype A). However, in another biopurification system setup, no such correlations were found. Our data suggest that in the simulated environmental settings, the occurrence of libA can be linked to the linuron mineralization capacity and that libA is primarily hosted by Variovorax phylotype A strains. However, the results also suggest that, apart from libA, other, as-yet-unknown isofunctional genes play an important role in linuron mineralization in the environment.
ISSN: 0099-2240
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division Soil and Water Management
Centre of Microbial and Plant Genetics
Sustainable Metals Processing and Recycling
Sustainable Chemical Process Technology TC, Technology Campus Diepenbeek
× corresponding author
# (joint) last author

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