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Title: Effects of C-reactive protein on human pulmonary vascular cells in chronic thromboembolic pulmonary hypertension
Authors: Wynants, Marijke *
Quarck, Rozenn *
Ronisz, Alicja
Alfaro-Moreno, Ernesto
Van Raemdonck, Dirk
Meyns, Bart
Delcroix, Marion # ×
Issue Date: Oct-2012
Publisher: Published jointly by the Society and Munksgaard
Series Title: European Respiratory Journal vol:40 issue:4 pages:886-894
Abstract: Chronic thromboembolic pulmonary hypertension (CTEPH) is characterised by proximal pulmonary vascular obstruction by thrombo-fibrotic material, the origin of which has not been elucidated. Enhanced inflammation could contribute to persistent obstruction by impairing pulmonary vascular cell function in CTEPH. We investigated C-reactive protein (CRP) effects on pulmonary vascular cell function in vitro. Primary cultures of proximal pulmonary endothelial cells (ECs) and smooth muscle cells (SMCs) from CTEPH and nonthromboembolic pulmonary hypertension (PH) patients were established. Recombinant CRP effects on mitogenic activity, adhesion capacity, endothelin-1 and von Willebrand factor (vWF) secretion and intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule-1 expression were investigated in ECs and/or SMCs. Expression of the CRP receptor, lectin-like oxidised low-density lipoprotein receptor (LOX)-1, was evaluated in proximal pulmonary arterial tissue and cells by Western blotting and immunofluorescence. CRP increased CTEPH-SMC proliferation by 250%. CRP increased adhesion capacity, endothelin-1 and vWF secretion by CTEPH-ECs by 37%, 129% and 694%, respectively. CRP-induced adhesion of CTEPH-ECs to monocytes was mediated by ICAM-1. CRP had no effect on cells from nonthromboembolic PH patients, probably because of overexpression of LOX-1 in CTEPH. Local expression of CRP was detected in ECs and SMCs within pulmonary arterial tissue. CRP may contribute to persistent obstruction of proximal pulmonary arteries in CTEPH by promoting vascular remodelling, endothelial dysfunction and in situ thrombosis.
ISSN: 0903-1936
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Cardiac Surgery
Facility for Surgery and Anaesthesiology
Experimental Thoracic Surgery
Pneumology
* (joint) first author
× corresponding author
# (joint) last author

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