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Title: Fluorescence imaging reveals the nuclear behavior of peroxisome proliferator-activated receptor retinoid X receptor heterodimers in the absence and presence of ligand
Authors: Feige, JN ×
Gelman, L
Tudor, Cicerone
Engelborghs, Yves
Wahli, W
Desvergne, B #
Issue Date: Jan-2005
Publisher: Amer soc biochemistry molecular biology inc
Series Title: Journal of Biological Chemistry vol:280 issue:18 pages:17880-17890
Abstract: In a global approach combining fluorescence recovery after photobleaching ( FRAP), fluorescence correlation spectroscopy (FCS), and fluorescence resonance energy transfer ( FRET), we address the behavior in living cells of the peroxisome proliferator-activated receptors (PPARs), a family of nuclear receptors involved in lipid and glucose metabolism, inflammation control, and wound healing. We first demonstrate that unlike several other nuclear receptors, PPARs do not form speckles upon ligand activation. The subnuclear structures that may be observed under some experimental conditions result from overexpression of the protein and our immunolabeling experiments suggest that these structures are subjected to degradation by the proteasome. Interestingly and in contrast to a general assumption, PPARs readily heterodimerize with retinoid X receptor (RXR) in the absence of ligand in living cells. PPAR diffusion coefficients indicate that all the receptors are engaged in complexes of very high molecular masses and/or interact with relatively immobile nuclear components. PPARs are not immobilized by ligand binding. However, they exhibit a ligand-induced reduction of mobility, probably due to enhanced interactions with cofactors and/or chromatin. Our study draws attention to the limitations and pitfalls of fluorescent chimera imaging and demonstrates the usefulness of the combination of FCS, FRAP, and FRET to assess the behavior of nuclear receptors and their mode of action in living cells.
URI: 
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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