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Title: A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes
Authors: Thimiri Govinda Raj, Deepak B.
Ghesquiere, Bart
Tharkeshwar, Arun Kumar
Coen, Katrijn
Derua, Rita
Vanderschaeghe, Dieter
Rysman, Evelien
Bagadi, Murali
Baatsen, Pieter
De Strooper, Bart
Waelkens, Etienne
Borghs, Gustaaf
Callewaert, Nico
Swinnen, Johan
Gevaert, Kris
Annaert, Wim # ×
Issue Date: Oct-2011
Publisher: Nature Pub. Group
Series Title: Molecular Systems Biology issue:7 pages:541
Article number: 541
Abstract: We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid isolation of plasma membranes (PMs), enabling high-resolution proteomic, glycomic and lipidomic analyses of the cell surface. We used this technology to characterize the effects of presenilin knockout on the PM composition of mouse embryonic fibroblasts. We found that many proteins are selectively downregulated at the cell surface of presenilin knockout cells concomitant with lowered surface levels of cholesterol and certain sphingomyelin species, indicating defects in specific endosomal transport routes to and/or from the cell surface. Snapshots of N-glycoproteomics and cell surface glycan profiling further underscored the power and versatility of this novel methodology. Since PM proteins provide many pathologically relevant biomarkers representing two-thirds of the currently used drug targets, this novel technology has great potential for biomedical and pharmaceutical applications. Molecular Systems Biology 7: 541; published online 25 October 2011; doi:10.1038/msb.2011.74
ISSN: 1744-4292
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory of Phosphoproteomics (-)
Laboratory of Membrane Trafficking
Clinical and Experimental Endocrinology
Semiconductor Physics Section
Laboratory of Lipid Metabolism and Cancer (+)
Laboratory of Protein Phosphorylation and Proteomics
× corresponding author
# (joint) last author

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