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Title: The Saccharomyces cerevisiae alcohol acetyl transferase Atf1p is localized in lipid particles
Authors: Verstrepen, Kevin ×
Van Laere, SDM
Vercammen, Jo
Derdelinckx, Guy
Dufour, JP
Pretorius, IS
Winderickx, Joris
Thevelein, Johan
Delvaux, Freddy #
Issue Date: Jan-2004
Publisher: John wiley & sons ltd
Series Title: Yeast vol:21 issue:4 pages:367-376
Abstract: The yeast alcohol acetyl transferase I, Atf1p, is responsible for the major part of volatile acetate ester production in fermenting Saccharomyces cerevisiae cells. Some of these esters, such as ethyl acetate and isoamyl acetate, are important for the fruity flavours of wine, beer and other fermented beverages. In order to reveal the subcellular localization of Atf1p and further unravel the possible physiological role of this protein, ATF1::GFP fusion constructs were overexpressed in brewer's yeast. The transformant strain showed a significant increase in acetate ester formation, similar to that of an ATF1 overexpression strain, indicating that the Atf1p-GFP fusion protein was active. UV fluorescence microscopy revealed that the fusion protein was localized in small, sphere-like organelles. These organelles could be selectively stained by the fluorescent dye Nile red, indicating that they contained high amounts of neutral lipids and/or sterols, a specific characteristic of yeast lipid particles. Purification of lipid particles from wild type and ATF1 deletion cells confirmed that the Atf1p-GFP fusion protein was located in these organelles. Furthermore, a clear alcohol acetyl transferase activity could be measured in the purified lipid particles of both wild type and transformed cells. The localization of Atf1p in lipid particles may indicate that Atf1p has a specific role in the lipid and/or sterol metabolism that takes place in these particles. Copyright (C) 2004 John Wiley Sons, Ltd.
URI: 
ISSN: 0749-503X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Centre for Food and Microbial Technology
Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Centre of Microbial and Plant Genetics
Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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