Congress of the European Society for Sexual Medicine edition:15 location:Amsterdam date:December 2012
We have recently demonstrated the essential role of adipose tissue-derived stem cell (ADSC) recruitment towards the major pelvic ganglion (MPG) in rats following cavernous nerve injury (CNI). The interaction between chemokines and their receptors plays a major role in this process. The objectives of this study were to examine chemokine subtype expression in neuro-inflammation of the rat MPG following cavernous nerve injury (CNI), and to evaluate the usefulness of tumor necrosis factor alpha (TNFA)-stimulated rat Schwann cells as an in-vitro model for neuronal chemokine production.
Six male 12 weeks old Sprague Dawley rats underwent laparotomy and bilateral crush injury of the cavernous nerves. Six rats served as sham controls (laparotomy and periprostatic dissection only). Twenty-four hours after CNI, the MPGs were harvested, RNA was isolated and subjected to qPCR analysis in triplicate. A rat peripheral nerve schwannoma derived Schwann cell line was acquired (RT4-D6P2T), and cultured in presence of TNFA in dosages of 0, 1, 10, and 100 nM for 24 hours in triplicate. Cells were then harvested and RNA was isolated and subjected to qPCR analysis.
Twenty-four hours following CNI, neuro-inflammation was present in the rat MPG as illustrated by significant upregulation of TNFA and transforming growth factor beta (TGFB) 1 and 2. Crush injury further resulted in significant upregulation of the chemokines CCL2-22-28, CXCL12, CX3CL1 and XCL1. The in-vitro stimulation of Schwann cells with TNFA mimicked this neuro-inflammatory condition at 24 hours as illustrated by a similar TGFB and chemokine RNA expression profile.
CNI-related neuro-inflammation in-vivo and in-vitro is accompanied by the expression of various chemokines. These chemokines may be responsible for the recruitment of ADSC towards the MPG following CNI in rats. In addition, we developed a Schwann cell culture model that can be employed to further study this recruitment in-vitro.