Title: Solution structure and conformational dynamics of deoxyxylonucleic acids (dXNA): An orthogonal nucleic acid candidate
Authors: Maiti, Mohitosh
Siegmund, Vanessa
Abramov, Mikhail
Lescrinier, Eveline
Rosemeyer, Helmut
Froeyen, Mathy
Ramaswamy, Amutha
Ceulemans, Arnout
Marx, Andreas
Herdewijn, Piet # ×
Issue Date: Jan-2012
Publisher: VCH Verlagsgesellschaft
Series Title: Chemistry - a European Journal vol:18 issue:3 pages:869-879
Article number: 10.1002/chem.201102509
Abstract: Orthogonal nucleic acids are chemically modified nucleic acid polymers that are unable to transfer information with natural nucleic acids and thus can be used in synthetic biology to store and transfer genetic information independently. Recently, it was proposed that xylose-DNA (dXNA) can be considered to be a potential candidate for an orthogonal system. Herein, we present the structure in solution and conformational analysis of two self-complementary, fully modified dXNA oligonucleotides, as determined by CD and NMR spectroscopy. These studies are the initial experimental proof of the structural orthogonality of dXNAs. In aqueous solution, dXNA duplexes predominantly form a linear ladderlike (type-1) structure. This is the first example of a furanose nucleic acid that adopts a ladderlike structure. In the presence of salt, an equilibrium exists between two types of duplex form. The corresponding nucleoside triphosphates (dXNTPs) were synthesized and evaluated for their ability to be incorporated into a growing DNA chain by using several natural and mutant DNA polymerases. Despite the structural orthogonality of dXNA, DNA polymerase β mutant is able to incorporate the dXNTPs, showing DNA-dependent dXNA polymerase activity.
ISSN: 0947-6539
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Department of Pharmaceutical & Pharmacological Sciences - miscellaneous
Quantum Chemistry and Physical Chemistry Section
× corresponding author
# (joint) last author

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