Title: Real-time monitoring of DNA hybridization and melting processes using a fiber optic sensor
Authors: Delport, Filip
Pollet, Jeroen
Janssen, Kris
Verbruggen, Bert
Knez, Karel
Spasic, Dragana
Lammertyn, Jeroen # ×
Issue Date: 2012
Publisher: IOP Pub.
Series Title: Nanotechnology vol:23 issue:6 pages:065503
Article number: 22248883
Abstract: In this paper a fiber optic surface plasmon resonance (FO-SPR) sensor was used to analyze the melting process of DNA linked to silica nanoparticles. Real-time monitoring of a DNA melting process has rarely been studied using surface plasmon resonance (SPR), since most commercial SPR setups do not allow for dynamic and accurate temperature control above 50 °C. The FO-SPR sensor platform, with silica nanobead signal amplification, allows sensing inside a standard PCR thermocycler, which makes high resolution DNA melting curve analysis possible. This innovative combination was used to characterize the hybridization and melting events between DNA immobilized on the sensor surface and DNA probes on silica nanoparticles. At optimized hybridization conditions complementary DNA strands of different length could be distinguished. While the real-time FO-SPR analysis of DNA hybridization did not result in significant variances, the analysis of DNA melting determined the exact length of overlap and the matching Gibbs energy.
ISSN: 0957-4484
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
× corresponding author
# (joint) last author

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