A new Kaliotoxin selective towards Kv1.3 and Kv1.2 but not Kv1.1 channels expressed in oocytes
Abbas, Najwa × Belghazi, Maya Abdel-Mottaleb, Yousra Tytgat, Jan Bougis, Pierre E Martin-Eauclaire, Marie-France #
Biochemical and Biophysical Research Communications vol:376 issue:3 pages:525-530
In this paper were described the purification, the sequencing, and the immunological biological characterization of a new Kaliotoxin analog, Aam-KTX, from the venom of the scorpion Androctonus amoreuxi The toxin effects on three cloned Kv channels (Kv1.1, Kv1.2, and Kv1.3) were investigated in Xenopus oocytes using electrophysiology experiments. The Aam-KTX preference for Kv1.3 channel versus Kv1.2 was expected (EC50 values, 1.1 +/- 0.02 and 10.4 +/- 1.5 nM, respectively) but its total inefficacity on Kv1.1 was very surprising. 3D molecular modeling of Aam-KTX brought putative answers to this difference in selectivity. (c) 2008 Elsevier Inc. All rights reserved.