A longer tracheal occlusion period results in increased lung growth in the nitrofen rat model
Beck, Veronica Davey, Markus G. Mayer, Steffi Froyen, Guy Deckx, Sebastiaan Klaritsch, Philipp Roubliova, Xenia I. Petersen, Scott G. Deprest, Jan A. # ×
John Wiley & Sons
Prenatal Diagnosis vol:32 issue:1 pages:39-44
Objective Prenatal tracheal occlusion (TO) promotes lung growth and is applied clinically in fetuses with severe
congenital diaphragmatic hernia. Limited data are available regarding the effect of duration of TO on lung
development. Our objective was to evaluate the effects of long (2 and 2.5 days) versus short (1 day) TO on lung
development in rats with nitrofen-induced diaphragmatic hernia.
Method Nitrofen was administered on embryonic day (ED) 9 and fetal TO performed either on ED18.5, 19 or 20
(term = 22 days). Sham-operated and untouched littermates served as controls. On ED21, lungs were harvested and
only fetuses with a left-sided diaphragmatic defect were included in further analyses.
Results Lung–body-weight ratio incrementally increased with the duration of TO. Increased proliferation following
long TO was confirmed by immunohistochemistry and qRT-PCR for the proliferation marker Ki-67. Irrespective of
duration, TO induced more complex airway architecture. Medial wall thickness of pulmonary arteries was thinner
after long rather than short TO.
Conclusion In the nitrofen rat model of congenital diaphragmatic hernia, a longer period of TO leads to enhanced
lung growth and less muscularized pulmonary arteries.