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Title: Reversible changes of the wheat gamma 46 gliadin conformation submitted to high pressures and temperatures
Authors: Lullien-Pellerin, V ×
Popineau, Y
Meersman, Filip
Morel, MH
Heremans, Karel
Lange, R
Balny, C #
Issue Date: Jan-2001
Publisher: Blackwell science ltd
Series Title: European Journal of Biochemistry vol:268 issue:22 pages:5705-5712
Abstract: The structure of the wheat gamma 46 gliadin was investigated, in aqueous solutions, under high pressure or temperature by the use of ultraviolet and fluorescence spectroscopic techniques. We found that high pressure (above 400 MPa) induces a change in the protein conformation that results in a decrease of the polarity of the environment of aromatic amino acids. This new conformation was able to bind the hydrophobic probe, 8-anilino-1-naphtalene-sulfonic acid (ANS), indicating an increase in the gliadin surface hydrophobicity. Thermodynamic parameters of this conformational change were measured and infrared spectroscopy studies were used to probe the potential secondary structure modifications. The high stability of gamma 46 gliadin could be related to its elastic character, as the observed changes were always found to be reversible.
URI: 
ISSN: 0014-2956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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