Title: Exploration of triple-helical fragments: crystallization and preliminary X-ray diffraction of d(TGGCCTTAAGG)
Authors: Van Hecke, Kristof ×
Uytterhoeven, Koen
Van Meervelt, Luc #
Issue Date: Jan-2007
Publisher: Blackwell publishing
Series Title: Acta crystallographica section f-structural biology and crystallization communications vol:63 pages:52-55
Abstract: The nonamer d(GCGAATTCG) and decamer d(GGCCAATTGG), containing one and two overhanging guanines, respectively, form G (.) GC triplets in their crystal packing. In order to introduce a third subsequent T (.) AT triplet, the decamer was further extended by one overhanging thymine residue. Two different crystal morphologies of the sequence d(TGGCCTTAAGG) were obtained by hanging-drop vapour diffusion and diffracted to 2.5 and 2.3 angstrom resolution, respectively. However, both crystals belong to the orthorhombic space group P2(1)2(1)2(1), with similar unit-cell parameters. Therefore, the two data sets could be merged to a resolution of 2.4 angstrom with unit-cell parameters a = 26.97, b = 41.12, c = 52.72 angstrom.
ISSN: 1744-3091
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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