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Title: A screening method for the simultaneous determination of putrescine, cadaverine, histamine, spermidine and spermine in fish by means of high pressure liquid chromatography of their 5-dimethylaminonaphthalene-1-sulphonyl derivatives
Authors: Rosier, Jan ×
Van Peteghem, C #
Issue Date: Jan-1988
Publisher: Springer-Verlag
Series Title: Zeitschrift für Lebensmittel-Untersuchung und -Forschung vol:186 issue:1 pages:25-8
Abstract: A simple and rapid method is described for the extraction, derivatization and subsequent determination by means of high pressure liquid chromatography of putrescine, cadaverine, histamine, spermidine and spermine as their 5-dimethylaminonaphthalene-1-sulphonyl derivatives. The amines are extracted from the fish material by an aqueous trichloroacetic acid solution and derivatized by means of 5-dimethylaminonaphthalene-1-sulphonylchloride (dansyl chloride). The reaction mixture is then injected on a RP-8 column using gradient elution to separate the amine derivatives. Use of the method results in a considerable saving with respect to both time and costs.
ISSN: 0044-3026
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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