Title: The role of local ROCK-inhibition in the pathogenesis of age-related macular degeneration
Authors: Hollanders, Karolien ×
Sijnave, Davine
Van Bergen, Tine
Van de Velde, Sarah
Vandewalle, Evelien
Moons, Lieve
Spileers, Werner
Stalmans, Ingeborg #
Issue Date: Oct-2011
Conference: EVER 2011 location:Hersonissos, Crete, Greece date:5-8 October 2011
Abstract: Purpose Age-related macular degeneration (AMD) is the leading cause of visual loss in
the elderly. ROCK inhibition may be a new way to treat angiogenesis-related disorders,
such as AMD correlated with choroidal neovascularization (CNV). " e use of the
ROCK-inhibition might have a therapeutic potential to inhibit angiogenesis, fi brosis
and infl ammation without causing severe side eff ects. " is project will investigate the
anti-angiogenic, anti-infl ammatory and anti-fi brotic effi cacy of ROCK-inhibition in a
mouse model of CNV.
Methods " ree laser spots of 50μm will be placed with a 532nm green laser around the
optic disc of C57BL/6J mice. Mice will be divided into two major experimental groups.
In one group the ROCK-inhibitor will be compared to a control solution (vehicle); in
the other group one eye will be injected with the murine anti-VEGF Ab and the other
eye with an irrelevant mouse Ab. All solutions will be injected intravitreally on day 0,
4 and 10. Mice will be sacrifi ced on diff erent time points after lasering. " e eff ects of
the ROCK-inhibitors will be checked on day 5 (infl ammation), day 14 (angiogenesis)
and day 35 (fi brosis). Expression of ROCK and VEGF in choroid and retina will be
determined using quantitative real-time RT PCR. Expression of ROCK-1 and ROCK-2
at the protein level will be confi rmed by Western Blot.
Conclusion AMD is the major cause of irreversible blindness worldwide. It is already
known that ROCK is associated with VEGF-driven angiogenesis. " erefore, the aim of
our project is fi rst to investigate the therapeutic potential of ROCK-inhibitors with a
local mode of action in the pathogenesis of CNV.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Ophthalmology
× corresponding author
# (joint) last author

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