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Title: Interaction of desacetamidocolchicine, a fast binding analog of colchicine with isotypically pure tubulin dimers alpha-beta(ii), alpha-beta(iii) and alpha-beta(iv)
Authors: Banerjee, A ×
Dhoore, A
Engelborghs, Yves #
Issue Date: Jan-1994
Publisher: Amer soc biochemistry molecular biology inc
Series Title: Journal of Biological Chemistry vol:269 issue:14 pages:10324-10329
Abstract: Desacetamidocolchicine (DAAC) is a colchicine analogue that lacks the acetamido side chain in the ring-B of colchicine. Unlike colchicine, it binds to tubulin very quickly, and yet it has powerful antimitotic properties. It has been demonstrated that the beta-tubulin isoforms differ significantly in their interactions with colchicine. In an effort to understand the role of the ring-B of colchicine, we have studied the interaction of DAAC with purified beta-tubulin isoforms. The association was studied fluorometrically using a stopped-now instrument under pseudo-first order conditions in the presence of a large excess of drug. The observed pseudo-first-order rate constants increased in a nonlinear way with the drug concentration, indicating that the binding of DAAC to tubulin isoforms occur in two steps as is true for the binding of colchicine to tubulin (Garland, D. L. (1978) Biochemistry 17, 4266-4272),
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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