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Title: MAPC Transplantation Confers a More Durable Benefit Than AC133(+) Cell Transplantation in Severe Hind Limb Ischemia
Authors: Aranguren, Xabier Lopez #
Pelacho, Beatriz
Penuelas, Ivan
Abizanda, Gloria
Uriz, Maialen
Ecay, Margarita
Collantaes, Maria
Arana, Miriam
Beerens, Manu
Coppiello, Giulia
Prieto, Ines
Perez-Ilzarbe, Maitane
Andreu, Enrique J
Luttun, Aernout
Prosper, Felipe # ×
Issue Date: 2011
Publisher: Cognizant Communication Corp.
Series Title: Cell Transplantation vol:20 issue:2 pages:259-269
Abstract: There is a need for comparative studies to determine which cell types are better candidates to remedy ischemia. Here, we compared human AC133(+) cells and multipotent adult progenitor cells (hMAPC) in a mouse model reminiscent of critical limb ischemia. hMAPC or hAC133(+) cell transplantation induced a significant improvement in tissue perfusion (measured by microPET) 15 days posttransplantation compared to controls. This improvement persisted for 30 days in hMAPC-treated but not in hAC133(+)-injected animals. While transplantation of hAC133(+) cells promoted capillary growth, hMAPC transplantation also induced collateral expansion, decreased muscle necrosis/fibrosis, and improved muscle regeneration. Incorporation of differentiated MC 133(+) or hMAPC progeny into new vessels was limited; however, a paracrine angio/arteriogenic effect was demonstrated in animals treated with hMAPC. Accordingly, hMAPC-conditioned, but not hAC133(+)-conditioned, media stimulated vascular cell proliferation and prevented myoblast, endothelial, and smooth muscle cell apoptosis in vitro. Our study suggests that although hAC133(+) cell and hMAPC transplantation both contribute to vascular regeneration in ischemic limbs, hMAPC exert a more robust effect through trophic mechanisms, which translated into collateral and muscle fiber regeneration. This, in turn, conferred tissue protection and regeneration with longer term functional improvement.
URI: 
ISSN: 0963-6897
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
× corresponding author
# (joint) last author

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