Title: Collective effects in individual oligomers of the red fluorescent coral protein DsRed
Authors: Cotlet, Mircea ×
Hofkens, Johan
Kohn, F
Michiels, Jan
Dirix, Gunter
Van Guyse, Maarten
Vanderleyden, Jozef
De Schryver, Frans #
Issue Date: Jan-2001
Publisher: Elsevier science bv
Series Title: Chemical Physics Letters vol:336 issue:5-6 pages:415-423
Abstract: We report on single-molecule experiments that were performed on two biological systems, the recently cloned red fluorescent protein of a coral of the Discosoma genus (DsRed) and the enhanced green fluorescent protein, both of which were immobilized in polyvinylalcohol. Fluorescence intensity trajectories were recorded and compared to those of a reference compound, the organic dye hexaphenylbenzene perylenemonoimide. Evidence was found for the presence of DsRed as an oligomer even at subnanomolar concentrations. Both proteins show triplet blinking in the millisecond time range. Collective effects could be observed for the Dsred oligomers. (C) 2001 Elsevier Science B.V. All rights reserved.
ISSN: 0009-2614
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Molecular Imaging and Photonics
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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