Title: High-resolution structure of a dna helix-forming (c-center-dot-g)asterisk-g base triplets
Authors: Van Meervelt, Luc ×
Vlieghe, D
Dautant, A
Gallois, B
Precigoux, G
Kennard, O #
Issue Date: Jan-1995
Publisher: Macmillan magazines ltd
Series Title: Nature vol:374 issue:6524 pages:742-744
Abstract: TRIPLE helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity, Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available, The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes(1,2) studied at about 3 Angstrom resolution, We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 Angstrom, in which the extended crystal structure contains (C . G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation, This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence, The present-structure agrees with many published predictions of tripler organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.
ISSN: 0028-0836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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