Title: Dynamics of flavin in flavocytochrome b(2): A fluorescence study
Authors: Albani, JR ×
Sillen, A
Engelborghs, Yves
Gervais, M #
Issue Date: Jan-1999
Publisher: Amer soc photobiology
Series Title: Photochemistry and photobiology vol:69 issue:1 pages:22-26
Abstract: The dynamics of the flavin bound to the flavocytochrome b(2) from Hansenula anomala were studied by fluorescence intensity quenching and quenching emission anisotropy with iodide, The fluorescence intensity of bound flavin is decreased 13-fold as compared to the free molecule. The remaining fluorescence decays with two lifetimes equal to 0.963 +/- 0.040 and 4.635 +/- 0.008 ns and fractional intensities of 0.036 +/- 0.002 and 0.964 +/- 0.002, respectively. The bimolecular diffusion constant was found to be 3.33 x 10(9) M-1 s(-1) when the flavin is bound to the enzyme and 8.3 x 10(9) M-1 s(-1) when the flavin is free in solution. Thus, the flavin in flavocytochrome b(2) is accessible to the solvent, but the amino acid residues of the binding site inhibit the diffusion of iodide, The rotational correlation time of bound flavin was found to be 2.015 +/- 0.365 ns, a value higher than that (155 ps) of free flavin in solution. Our results are discussed on the basis of local dynamics of the flavin.
ISSN: 0031-8655
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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