Communications in Agricultural and Applied Biological Sciences vol:76 issue:1 pages:125-128
A major difficulty in the battle against Salmonella is the fact that Salmonella can form biofilms on various surfaces. This is an important survival strategy, both in- and outside the host. Therefore, the prevention and/or eradication of biofilms could be an effective way to limit the spread of Salmonella.
We conducted a high-throughput screening of 20.014 small molecules, in search of Salmonella biofilm inhibitors with a broad (temperature) activity range and therefore have potential to be used both in- and outside the host. We aim at identifying compounds that inhibit biofilm formation, without killing the bacteria, so the development of resistance is less likely.
We identified 144 (0.7%) possible biofilm inhibitors. The dose-response relationship of these was determined, as well as the effect on the planktonic growth. The compounds with maximum biofilm inhibitory activity and minimal effect on planktonic growth were studied further, both on prevention and destruction of biofilms from Salmonella and Pseudomonas.
This way we identified 9 ‘lead-families’, from which analogues were purchased. Using these the “structure-activity relationship” is determined. This will yield several ‘lead-compounds’ from which the activity in different in vitro and in vivo test systems will be determined, as well as the ‘Mode of Action’.