Nuclear factor-kappaB signature of inflammatory breast cancer by cDNA microarray validated by quantitative real-time reverse transcription-PCR, immunohistochemistry, and nuclear factor-kappaB DNA-binding
Van Laere, Steven × Van der Auwera, Ilse Van den Eynden, Gert G Elst, Hilde J Weyler, Joost Harris, Adrian L van Dam, Peter Van Marck, Eric A Vermeulen, Peter B Dirix, Luc Y #
Association for Cancer Research
Clinical Cancer Research vol:12 issue:11 Pt 1 pages:3249-56
Inflammatory breast cancer (IBC) is the most aggressive form of locally advanced breast cancer with high metastatic potential. In a previous study, we showed that IBC is a different form of breast cancer compared with non-IBC by cDNA microarray analysis. A list of 756 genes with significant expression differences between IBC and non-IBC was identified. In-depth functional analysis revealed the presence of a high number of nuclear factor-kappaB (NF-kappaB) target genes with elevated expression in IBC versus non-IBC. This led to the hypothesis that NF-kappaB contributes to the phenotype of IBC. The aim of the present study was to further investigate the role of NF-kappaB in IBC.