Title: Inhibition of human interferon-gamma biosynthesis by an antisense RNA-expressing vector
Authors: Boeve, CMA ×
Wyngaerd, AV
De Ley, Marc #
Issue Date: Jan-1996
Publisher: Williams & wilkins
Series Title: Molecular Pharmacology vol:49 issue:1 pages:58-62
Abstract: We applied antisense RNA technology for reducing the level of human IFN-gamma (HuIFN-gamma) expression. An antisense RNA vector containing the full-length HuIFN-gamma cDNA in the opposite orientation was electroporated into cells constitutively producing very high levels of the cytokine. Approximately 53% of the resulting clones exhibited a specific HuIFN-gamma inhibition of an average of 95.5%. The results of reverse transcription-polymerase chain reaction and Northern blot analyses revealed that the antisense effect originated from a specific reduction of the targeted mRNA caused by antisense RNA expression. This very effective antisense RNA strategy can have possible therapeutic applications in treating diseases where HuIFN-gamma is known to play a negative role, such as in certain autoimmune diseases.
ISSN: 0026-895X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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