Bulletin de la Société Belge d'Ophtalmologie vol:317 pages:65-66
Ophthalmologia Belgica Congress location:Brussels, Belgium date:23-25 November 2011
PURPOSE The aim of our study was to check the hypothesis that placental
growth factor (PlGF) plays a role in scar formation after glaucoma filtration
surgery (GFS), and that it may be a target for improvement of the outcome
of this surgery.
METHODS Since the rabbit is the most popular animal used for investigating
postoperative scar formation, the cross-reactivity to rabbit-PlGF with human
and murine PlGF-antibodies (16D3 and 5D11D4; ThromboGenics NV) was
tested using ELISA. Murine conjunctival fibroblasts were isolated and
cultured. The purity of the cultured cells was verified by an immunostaining
for vimentin. Also, a mouse model of GFS was set-up, according to the
technique Seet L, et al. (Mol Med 2011).
RESULTS ELISA-experiments demonstrated that none of the PlGFantibodies
showed cross-reactivity to rabbit PlGF. So, these antibodies could
not be used in the standard rabbit model of glaucoma filtration surgery.
Therefore, a mouse model of GFS was set-up and different immunostainings
showed an increase of angiogenesis and inflammation during the initial
phase of healing and fibrosis at the later stages. Cultured murine
conjunctival fibroblasts were all positive for vimentin, which confirmed the
purity of the fibroblast culture.
CONCLUSION Future experiments will elucidate the in vitro and in vivo role
of the murine PlGF-antibody (5D11D4; ThromboGenics NV) on cultured
fibroblasts and in the mouse model of GFS. This research project will clarify
the potential role of PlGF-inhibition in the improvement of filtration surgery
outcome, and will highlight any angiostatic, anti-inflammatory, and/or