Title: Evaluation of a new polymeric stationary phase with reversed-phase properties for high temperature liquid chromatography
Authors: Vanhoenacker, Gerd
Dos Santos Pereira, Alberto
Kotsuka, Takashi
Cabooter, Deirdre
Desmet, Gert
Sandra, Pat # ×
Issue Date: May-2010
Publisher: Elsevier
Series Title: Journal of Chromatography A vol:1217 issue:19 pages:3217-3222
Conference: 34th International Symposium on High Performance Liquid Phase Separations and Related Techniques Dresden, GERMANY, JUN 28-JUL 02, 2009
Abstract: The performance of a polymeric stationary phase with reversed-phase properties (ET-RP1) was evaluated for LC separations at elevated temperature. The most significant observation was that the reduced plate height (h) decreased from 3.4 at 25 degrees C (optimal flow 0.5 mL/min) to 2.4 at 150 degrees C (optimal flow 2.5 mL/min) which is comparable to the efficiency obtained with silica-based reversed-phase columns of 4.6mm ID operated at 0.8 mL/min. The phase showed no deterioration after long use at 150 degrees C within the pH range 1-9. Catalytic activity originating from the stationary phase material, e.g. as experienced on zirconium columns operated at elevated temperature, was absent. The performance of ET-RP1 is illustrated with the analysis of some pharmaceutical samples by LC and LC-MS. Operation at elevated temperature also allows to reduce the amount of organic modifier or to replace acetonitrile and methanol by the biodegradable ethanol.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science