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Title: The kinetics of heat-induced structural changes of beta-lactoglobulin
Authors: Sava, N ×
Van der Plancken, Iesel
Claeys, Wendie
Hendrickx, Marc #
Issue Date: Jan-2005
Publisher: Amer dairy science assoc
Series Title: Journal of dairy science vol:88 issue:5 pages:1646-1653
Abstract: Heat-induced structural changes of beta-lactoglobulin were studied at temperatures ranging from 67.5 to 82.5 degrees C, and at pH 7.5. These changes were monitored by measurement of surface hydrophobicity, thiol availability, and protein solubility. Kinetic studies were conducted to quantitatively describe the contribution of hydrophobic and SH/SS interchange reactions to the thermal structural changes of beta-lactoglobulin. Results indicate that beta-lactoglobulin is sensitive to heat-induced interchange reactions with consequences for protein solubility. The extent of changes measured by the increase in surface hydrophobicity and the decrease in slow-reacting SH groups content could be described by a first-order fractional conversion model and were characterized by activation energy values of 233.9 +/- 8.6 and 148.2 +/- 6.7 kJ/mol, respectively. The break in the Arrhenius plot suggested in literature for beta-lactoglobulin denaturation was confirmed in this study only for the kinetics of exposed SH groups.
ISSN: 0022-0302
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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