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Title: Method to predict and compare the influence of the particle size on the isocratic peak capacity of high-performance liquid chromatography columns
Authors: Cabooter, Deirdre
de Villiers, Andre
Clicq, David
Szucs, Roman
Sandra, Pat
Desmet, Gert # ×
Issue Date: Apr-2007
Publisher: Elsevier
Series Title: Journal of Chromatography A vol:1147 issue:2 pages:183-191
Abstract: A kinetic plot based method has been used to experimentally predict the optimal particle size yielding the maximal isocratic peak capacity in a given analysis time. Applying the method to columns of three different manufacturers and characterizing them by separating a 4-component paraben mixture at 30 degrees C, it was consistently found that the classical 3 and 3.5 mu m particles provide the highest peak capacity for typical isocratic separation run times between 30 and 60 min when operating the columns at a conventional pressure of 400 bar. Even at 1000 bar, the sub-2 mu m particles only have a distinct advantage for runs lasting 30 min or less, while for runs lasting 45 min or longer the 3 and 3.5 mu m again are to be preferred. This finding points at the advantage for high-resolution separations that could be obtained by producing 3 and 3.5 mu m particle columns that can be operated at elevated pressures. (c) 2007 Elsevier B.V. All rights reserved.
URI: 
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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