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Title: A study of the parameters affecting the accuracy of the total pore blocking method
Authors: Liekens, Anuschka ×
Cabooter, Deirdre
Denayer, Joeri
Desmet, Gert #
Issue Date: Oct-2010
Publisher: Elsevier
Series Title: Journal of Chromatography A vol:1217 issue:43 pages:6754-6761
Abstract: We report on a study wherein we investigate the different factors affecting the accuracy of the total pore blocking method to determine the interstitial volume of reversed-phase packed bed columns Octane. nonane, decane and dodecane were all found to be suitable blocking agents, whereas heptane already dissolves too well in the applied fully aqueous buffers The method of moments needs to be used to accurately determine the elution times, and a proper correction for the frit volume is needed. Failing to do so can lead to errors on the observed interstitial volume of the order of 2% or more It has also been shown that the application of a high flow rate or a high pressure does not force the blocking agent out of the mesopores of the particles. The only potential source of loss of blocking agent is dissolution into the mobile phase (even though this is a buffered fully aqueous solution). This effect however only becomes significant after the elution of 400 geometrical column volumes, i e, orders more than needed for a regular total pore blocking experiment (C) 2010 Elsevier B.V. All rights reserved
URI: 
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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