Title: Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's
Authors: Francois, Isabelle ×
Cabooter, Deirdre
Sandra, Koen
Lynen, Frédéric
Desmet, Gert
Sandra, Pat # ×
Issue Date: Apr-2009
Publisher: Wiley-VCH Verlag GMBH
Series Title: Journal of Separation Science vol:32 issue:8 pages:1137-1144
Abstract: As an alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LCxRP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.
ISSN: 1615-9306
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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