Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's
Francois, Isabelle × Cabooter, Deirdre Sandra, Koen Lynen, Frédéric Desmet, Gert Sandra, Pat # ×
Wiley-VCH Verlag GMBH
Journal of Separation Science vol:32 issue:8 pages:1137-1144
As an alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LCxRP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.