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Title: Determination of the ground-state dissociation-constant by fluorometric titration
Authors: Kowalczyk, A ×
Boens, Noël
Vandenbergh, V
Deschryver, Fc #
Issue Date: Jan-1994
Publisher: Amer chemical soc
Series Title: Journal of physical chemistry vol:98 issue:34 pages:8585-8590
Abstract: The fluorimetric determination of the ground-state dissociation constant K-d of intermolecular bicompartmental systems in the absence and presence of an excited-state reaction is discussed. The presence of an excited-state reaction can result in very complicated fluorimetric titration curves, precluding the evaluation of K-d. The interference of the excited-state reaction can be circumvented if the fluorimetric titration is performed at the isoemissive point. Under this condition, the correct value of K-d Will be obtained from the unique inflection point of the titration curve. It is further shown that previous recommendations to excite either at the isosbestic point or at a wavelength where only one species absorbs are inappropriate.
ISSN: 0022-3654
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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