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Title: Characterization of the structure and conformation of platelet-derived growth factor-bb (pdgf-bb) and proteinase-resistant mutants of pdgf-bb expressed in saccharomyces-cerevisiae
Authors: Craig, S ×
Clements, Jm
Cook, Al
Dryden, Dtf
Green, Dr
Heremans, Karel
Kirwin, Pm
Price, Mj
Fallon, A #
Issue Date: Jan-1992
Publisher: Portland press
Series Title: Biochemical journal vol:281 pages:67-72
Abstract: A detailed biophysical study of the secondary and tertiary structures of recombinant platelet-derived growth factor (PDGF)-BB produced in yeast has been carried out. The secondary structure of the molecule is composed of 54 % beta-sheet with less than 5 % ordered helix. The single tryptophan residue has been shown to be solvent-accessible; however, the ability of the side chain to rotate is severely restricted. The fluorescence emission is quenched at pH 7.0 and in the presence of high salt, but dequenched by titration to lower pH with a pK of 5.8. Two proteinase-resistant mutants of PDGF ([Ser28]- and [Pro32]-PDGF-BB) have also been characterized and shown to have secondary and tertiary structures indistinguishable from wild-type PDGF-BB. These are, therefore, suitable stable background molecules in which to carry out structure-activity-relationship studies on PDGF-BB.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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