Title: Two-dimensional Fourier-transform infrared correlation spectroscopy study of the high-pressure tuning of proteins
Authors: Smeller, L ×
Rubens, P
Frank, J
Fidy, J
Heremans, Karel #
Issue Date: Jan-2000
Publisher: Elsevier science bv
Series Title: Vibrational spectroscopy vol:22 issue:1-2 pages:119-125
Abstract: 2D FTIR gives new information about the pressure effect on the structure and dynamics of macromolecular systems, Application of this analysis to proteins can unravel the relation of conformational changes and H/D exchange processes. For lipoxygenase; a pressure of 6.5 kbar induces irreversible conformational changes resulting in an increased exposure of interior parts of the protein to the solvent. At the transition pressure the spectral changes indicate a correlation between conformational changes and H/D exchange. Below and above this pressure, the effects of H/D exchange on the spectral changes are predominant. (C) 2000 Elsevier Science B.V. All rights reserved.
ISSN: 0924-2031
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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