Title: Two-dimensional correlation infrared spectroscopic study on the conformational changes occurring in the thermally induced pretransition of ribonuclease A
Authors: Wang, Li-Xu ×
Wu, Yuqing
Meersman, Filip #
Issue Date: Jan-2006
Publisher: Elsevier science bv
Series Title: Journal of Molecular Structure vol:799 issue:1-3 pages:85-90
Abstract: Thermal unfolding of ribonuclease A (RNase A) in deuterated Tris buffer is studied by Fourier transform infrared (FT-IR) spectroscopy. Two kinds of two-dimensional (2D) correlation spectroscopy, variable variable (VV) 2D and sample-sample (SS) 2D spectroscopy, have been employed to analyze the observed thermally induced spectral variations of RNase A. Using SS 2D spectroscopy one can observe a pretransition at 45 degrees C prior to the main transition at 66 degrees C. This pretransition cannot be detected by a single-frequency analysis or the SS 2D correlation analysis of the original infrared spectra of RNase A. The VV 2D correlation spectroscopy study provides information about the structural changes that occur during these transitions: in the pretransition, the observed structural variations are associated with local conformational changes of an alpha-helical segment and the modification of a certain amount of beta-sheet structure; in the main unfolding, changes in irregular and alpha-helical structures are followed by those in the beta-sheet structure, including the antiparallel beta-sheet components, resulting in the loss of secondary structure. This work demonstrates that 2D correlation spectroscopy can be used as an alternative to principal component analysis. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 0022-2860
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science