Title: Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov.
Authors: Justé, Annelies ×
Van Trappen, Stefanie
Verreth, Christel
Cleenwerck, Ilse
De Vos, Paul
Lievens, Bart
Willems, Kris #
Issue Date: 2012
Publisher: Society for General Microbiology
Series Title: International Journal of Systematic and Evolutionary Microbiology vol:62 issue:1 pages:129-137
Abstract: Most bacteria recovered so far from sugar thick juice during storage represent strains of the
species Tetragenococcus halophilus. Recently, several Gram-positive, non-motile, non-sporeforming
cocci with other physiological and genetic traits were isolated from sugar thick juice
samples from different origins. In this study, representative isolates were investigated using a
polyphasic taxonomic approach. The 16S rRNA gene sequence similarity between these isolates
and their closest relative, Tetragenococcus muriaticus, was 97.4 %. The level of DNA–DNA
relatedness between isolate T1T, representing the newly found Tetragenococcus isolates, and T.
muriaticus was 57 %. Isolate T1T had a DNA G+C content of 36.7 mol%. Phylogenetic data and
genomic and phenotypic features demonstrated that the isolates represent a novel species, for
which the name Tetragenococcus osmophilus sp. nov. is proposed with T1T as the type strain
(LMG 26041T 5DSM 23765T). Additionally, T. halophilus isolates from high-salt and highsugar
environments showed clear differences in several physiological and genetic characteristics
like RAPD fingerprints and 16S rRNA gene sequences. DNA–DNA hybridizations, however,
showed 79 to 80% relatedness between osmophilic and halophilic T. halophilus isolates,
demonstrating that the different strains belong to the same species. Based on the phenotypic and
genotypic differences observed, as well as the different origins of the strains and the industrial
relevance of thick juice degradation, two subspecies of T. halophilus are described in this
manuscript: T. halophilus subsp. halophilus subsp. nov. for the strains isolated from salt media
and T. halophilus subsp. flandriensis subsp. nov. for the strains isolated from sugar-rich
environments, which were first isolated in Flanders, Belgium. The type strains for the subspecies
are IAM 1676T (5LMG 11490T 5DSM 20339T) and T5T (5LMG 26042T 5DSM 23766T),
ISSN: 1466-5026
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Microbial and Molecular Systems - miscellaneous
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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