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Title: Development of a DNA array for the simultaneous detection and identification of sugar thick juice bacterial contaminants
Authors: Justé, Annelies
Lievens, Bart ×
Frans, Ingeborg
Klingeberg, M
Michiels, Chris
Willems, Kris #
Issue Date: Jun-2011
Publisher: Springer
Series Title: Food Analytical Methods vol:4 issue:2 pages:173-185
Abstract: Despite the use of generally accepted good
storage practices, sugar thick juice degradation caused by
microbiological contamination occasionally occurs, causing
considerable financial loss. In this study, a DNA array was
developed for simultaneous detection and identification of
the most prominent microflora present during thick juice
storage, which may cause degradation of the thick juice.
Specific oligonucleotides were developed for several
bacterial taxa, including the genera Bacillus, Kocuria,
Staphylococcus and Tetragenococcus and the species
Aerococcus viridans, Leuconostoc mesenteroides and Tetragenococcus
halophilus. The DNA array was validated
using both pure cultures and industrial samples. In addition,
comparisons were made between the developed array, PCR
assays specifically targeting the thick juice contaminants
and classical microbial platings. The array was found to be
reliable and sensitive enough to detect and identify the
target bacteria. In addition, the array was used to monitor
the target microbial populations in thick juice during longterm
storage and degradation. Results are discussed in
relation to DNA stability in thick juice.
ISSN: 1936-9751
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Technologiecluster Bioengineering Technologie
Bioengineering Technology TC @ Thomas More Mechelen
Microbial and Molecular Systems - miscellaneous
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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