ITEM METADATA RECORD
Title: Modulation of human interferon-gamma biosynthesis by antisense oligodeoxynucleotides
Authors: Boeve, CMA ×
De Ley, Marc #
Issue Date: Jan-2000
Publisher: Humana press inc
Series Title: Molecular biotechnology vol:14 issue:2 pages:157-163
Abstract: We investigated the inhibition of human interferon-gamma (HuIFN-gamma) production in cultures of lymphocytes with the use of the antisense strategy. Out of a series of antisense oligodeoxynucleotides (ODN) complementary to different regions of the HuIFN-gamma gene, a 16-mer specific for a sequence including the translation initiation codon was the most effective. Here we describe a detailed protocol for the isolation of lymphocytes fi om buffy cents, the rational design of antisense ODN, and the monitoring nf HuIFN-gamma production of antisense ODN-treated cells.
ISSN: 1073-6085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy

 




All items in Lirias are protected by copyright, with all rights reserved.

© Web of science