Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)
Studer, Bruno × Asp, Torben Frei, Ursula Hentrup, Stephan Meally, Helena Guillard, Aurelie Barth, Susanne Muylle, Hilde Roldan Ruiz, Isabel Barre, Philippe Koning-Boucoiran, Carole Uenk-Stunnenberg, Gerda Dolstra, Oene Skot, Leif Skot, Kirsten P Turner, Lesley B Humphreys, Mervyn O Kolliker, Roland Roulund, Niels Nielsen, Klaus K Lubberstedt, Thomas #
Kluwer Academic Publishers
Molecular Breeding vol:21 issue:4 pages:533-548
An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.