Title: Isothermal titration calorimetry and surface plasmon resonance allow quantifying substrate binding to different binding sites of Bacillus subtilis xylanase
Authors: Cuyvers, Sven ×
Dornez, Emmie
Abou Hachem, Maher
Svensson, Birte
Hothorn, Michael
Chory, Joanne
Delcour, Jan
Courtin, Christophe #
Issue Date: Jan-2012
Publisher: Elsevier
Series Title: Analytical Biochemistry vol:420 issue:1 pages:90-92
Abstract: Isothermal titration calorimetry and surface plasmon resonance were tested for their ability to study substrate binding to the active site (AS) and to the secondary binding site (SBS) of Bacillus subtilis xylanase A separately. To this end, three enzyme variants were compared. The first was a catalytically incompetent enzyme that allows substrate binding to both the AS and SBS. In the second enzyme, binding to the SBS was impaired by site-directed mutagenesis, whereas in the third enzyme, the AS was blocked using a covalent inhibitor. Both techniques were able to show that AS and SBS have a similar binding affinity.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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