Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices
Papazova, Nina × Ghedira, Rim Van Glabeke, Sabine Bartegi, Aghleb Windels, Pieter Taverniers, Isabel Roldan Ruiz, Isabel Van Bockstaele, Erik Milcamps, Anne Van den Eede, Guy Depicker, Ann De Loose, Marc #
Plant Cell Reports vol:27 issue:4 pages:749-757
Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.