Title: Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices
Authors: Papazova, Nina ×
Ghedira, Rim
Van Glabeke, Sabine
Bartegi, Aghleb
Windels, Pieter
Taverniers, Isabel
Roldan Ruiz, Isabel
Van Bockstaele, Erik
Milcamps, Anne
Van den Eede, Guy
Depicker, Ann
De Loose, Marc #
Issue Date: Apr-2008
Publisher: Springer
Series Title: Plant Cell Reports vol:27 issue:4 pages:749-757
Abstract: Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.
ISSN: 0721-7714
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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