Title: A novel hydrolase identified by genomic-proteomic analysis of phenylurea herbicide mineralization by Variovorax sp. strain SRS16
Authors: Bers, Karolien
Leroy, Baptiste
Breugelmans, Philip
Albers, Pieter
Lavigne, Rob
Sørensen, Sebastian R
Aamand, Jens
De Mot, René
Wattiez, Ruddy
Springael, Dirk # ×
Issue Date: Dec-2011
Publisher: American Society for Microbiology (ASM)
Series Title: Applied and Environmental Microbiology vol:77 issue:24 pages:8754-8764
Abstract: The soil bacterial isolate Variovorax sp. strain SRS16 mineralizes the phenylurea herbicide linuron. The proposed pathway initiates with hydrolysis of linuron to 3,4-dichloroaniline (DCA) and N,O-dimethylhydroxylamine, followed by conversion of DCA to Krebs cycle intermediates. Differential proteomic analysis showed a linuron-dependent up-regulation of several enzymes that fit into this pathway including an amidase (LibA), a multicomponent chloroaniline dioxygenase and enzymes associated with a modified chlorocatechol ortho-cleavage pathway. Purified LibA is a monomeric linuron hydrolase of ∼55 kDa with a K(M) and V(max) for linuron of 5.8 μM and 0.16 nmol min(-1), respectively. This novel member of the Amidase Signature family is unrelated to phenylurea-hydrolyzing enzymes from Gram-positive bacteria and lacks activity towards other tested phenylurea herbicides. Orthologues of libA are present in all other tested linuron-degrading Variovorax strains with the exception of strains Variovorax WDL1 and Variovorax PBS-H4, suggesting divergent evolution of the linuron catabolic pathway in different Variovorax strains. The organization of the linuron degradation genes identified in the draft SRS16 genome sequence indicates that gene patchwork assembly is at the origin of the pathway in SRS16. Transcription analysis suggests that a catabolic intermediate, rather than linuron itself, acts as effector in activation of the pathway. Our study provides the first report on the genetic organization of a bacterial pathway for complete mineralization of a phenylurea herbicide and the first report on a linuron hydrolase in Gram-negative bacteria.
ISSN: 0099-2240
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Division Soil and Water Management
Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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