Title: Response of Mouse Brain Perfusion to Hypo- and Hyperventilation Measured by Arterial Spin Labeling
Authors: Oosterlinck, Wouter ×
Dresselaers, Tom
Geldhof, V
Van Santvoort, A
Robberecht, Wim
Herijgers, Paul
Himmelreich, Uwe #
Issue Date: Sep-2011
Publisher: Wiley-Liss
Series Title: Magnetic Resonance in Medicine vol:66 issue:3 pages:802-811
Abstract: We aimed to setup a noninvasive and well-controlled methodology for evaluation of the cerebrovascular response in mice (C57BL/6J; 12 weeks). Therefore we applied a normo-, hypo-, and hyperventilation paradigm combined with arterial spin labeling and monitoring of the expired CO2 (expCO(2)) (n = 7) or arterial pCO(2) (apCO(2)) (n = 12). Reducing the tidal volume by 25% and the respiratory rate by 20% resulted in hypercapnia (apCO(2) from 33 +/- 6 mmHg to 64 +/- 16 mmHg). Increasing the respiratory rate by 25% and the tidal volume by 20% decreased apCO(2) to 22 +/- 5 mmHg. Cerebral blood flow (CBF) was 82 +/- 21, 163 +/- 41 and 64 +/- 18 mL/100 g/min during normo, hypo-, and hyperventilation, respectively (midbrain). The correlation of apCO(2) and CBF levels resulted in a cerebrovascular response of 2.7 +/- 0.3, 2.1 +/- 0.3, 2.1 +/- 0.3, and 3.7 +/- 0.5 mL/100 g/min/mmHg for midbrain, cortex, hippocampus and thalamus, respectively. As expCO2 levels were correlated with apCO(2) (r(2) = 0.86; n = 4) and CBF (r(2) = 0.67) a cerebrovascular response based on simultaneously recorded CBF and expCO(2) levels could be derived (3.3 +/- 0.5, 2.5 +/- 0.4, 3.0 +/- 0.4, and 4.5 +/- 0.6 mL/100 g/min/mmHg; order as above). A cross-over experiment resulted in similar responses. In conclusion, this protocol allows evaluating basal CBF and cerebrovascular response in mice under well-controlled conditions by simply changing ventilator settings and correlating CBF with apCO(2) and/or simultaneously obtained expCO(2). Magn Reson Med 66:802-811, 2011. (C) 2011 WileyLiss, Inc.
ISSN: 0740-3194
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Experimental Cardiac Surgery (-)
Biomedical MRI
Research Group Experimental Neurology
Laboratory for Neurobiology (VIB-KU Leuven Center for Brain & Disease Research)
× corresponding author
# (joint) last author

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