Title: Multi-level factorial analysis of Ca2+/Pi supplementation as bio-instructive media for in vitro biomimetic engineering of three-dimensional osteogenic hybrids
Authors: Chai, Yoke Chin
Roberts, Scott
Van Bael, Simon
Chen, Yantian
Luyten, Frank
Schrooten, Jan # ×
Issue Date: Feb-2012
Publisher: Mary Ann Liebert, Inc. Publishers
Series Title: Tissue Engineering Part C, Methods vol:18 issue:2 pages:90-103
Abstract: We report on the in vitro use of Ca2+/Pi supplementation as a bio-instructive medium to drive human periosteum-derived cells (hPDCs) towards osteogenic differentiation on three-dimensional (3D) porous Ti6Al4V scaffolds. Through a multi-level factorial analysis, we have investigated systematically the biological effect and interactions of Ca2+ or Pi supplementation in three selected media preparations [i.e. basic growth medium (GM), osteogenic medium (OM) and osteogenic medium without β-glycerophosphate (OM-)] and have identified specific conditions which induce proliferation and significant osteogenic differentiation of 2D hPDC cultures. These findings were translated from 2D to 3D cultures conditions to instruct hPDCs to populate porous Ti6Al4V scaffolds and to differentiate into the osteoblast lineage with collagenous matrix production and subsequent matrix mineralisation on the 3D structures. These osteogenic hybrids may potentially serve as a clinically-relevant customisable bone reparative unit, providing a biomimetic template to more effectively mediate in vivo bone regeneration.
ISSN: 1937-3384
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Production Engineering, Machine Design and Automation (PMA) Section
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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