Title: Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium
Authors: Gross, Gabriele ×
van der Meulen, Jan
Snel, Johannes
van der Meer, Roelof
Kleerebezem, Michiel
Niewold, Theo
Hulst, Marcel M
Smits, Mari A #
Issue Date: Nov-2008
Publisher: Elsevier Science Publishers, B.V. on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Immunology and Medical Microbiology vol:54 issue:2 pages:215-223
Abstract: Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
ISSN: 0928-8244
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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