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Title: A standardized and biocompatible preparation of aggregate-free amyloid beta peptide for biophysical and biological studies of Alzheimers disease
Authors: Broersen, Kerensa
Jonckheere, Wim
Rozenski, Jef
Vandersteen, Annelies
Pauwels, Kris
Pastore, Annalisa
Rousseau, Frederic
Schymkowitz, Joost # ×
Issue Date: Sep-2011
Publisher: Oxford University Press
Series Title: Protein Engineering, Design & Selection vol:24 issue:9 pages:743-750
Abstract: We provide a validated and rapid protocol for the solubilization of amyloid -peptide (A). This procedure involves sequential solubilization using structure-breaking organic solvents hexafluoroisopropanol and DMSO followed by column purification. The low solubility and tendency of A to aggregate considerably impede the in vitro handling and biophysical or biological investigation of A, despite the interest in this peptide because of its implication in Alzheimers disease. The main advantage of the proposed protocol over others is that it results in standardized aggregate-free A peptide samples that are biocompatible for cell culture studies and yield reproducible aggregation kinetics and cytotoxicities. This three-step protocol also enables the co-solubilization of the longer A42 variant with A40 in ratios relevant to Alzheimers disease.
ISSN: 1741-0126
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Switch Laboratory
× corresponding author
# (joint) last author

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