Title: Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues
Authors: Vondenhoff, Gaston H. M
Blanchaert, Bart
Geboers, Sophie
Kazakov, Teymur
Datsenko, Kirill A
Wanner, Barry L
Rozenski, Jef
Severinov, Konstantin
Van Aerschot, Arthur # ×
Issue Date: Jul-2011
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Bacteriology vol:193 issue:14 pages:3618-3623
Abstract: Microcin C (McC), a natural antibacterial compound consisting of a heptapeptide attached to a modified adenosine, is actively taken up by the YejABEF transporter, after which it is processed by cellular aminopeptidases, releasing the nonhydrolyzable aminoacyl adenylate, an inhibitor of aspartyl-tRNA synthetase. McC analogues with variable length of the peptide moiety were synthesized and evaluated in order to characterize the substrate preferences of the YejABEF transporter. It was shown that a minimal peptide chain length of 6 amino acids and the presence of an N-terminal formyl-methionyl-arginyl sequence are required for transport.
ISSN: 0021-9193
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Pharmaceutical Analysis
Drug Delivery and Disposition
× corresponding author
# (joint) last author

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